Chelsea announced the signing of David Luiz from Paris St Germain on a three-year contract in the top transfer of deadline day, adding to the signing of Marcos Alonso from Fiorentina.
Meanwhile, France midfielder Moussa Sissoko confirmed he had signed for Tottenham on a five-year contract from Newcastle via social media in the day's other big deal.
Earlier, Joe Hart, Wilfried Bony, Samir Nasri and Eliaquim Mangala all left Manchester City on season-long loans as Pep Guardiola trimmed his squad on transfer deadline day.
England goalkeeper Hart completed his anticipated move to Torino, while striker Bony, playmaker Nasri and defender Mangala left for Stoke, Sevilla and Valencia respectively.
Werder Bremen completed the signing of Serge Gnabry from Arsenal for an undisclosed fee.
The 21-year-old passed his medical on Wednesday morning after being released by Germany's under-21s to agree personal terms with the northern Germany club.
Arsenal also allowed Jack Wilshere to go out on a season-long loan deal to Bournemouth.
Reigning Premier League champions Leicester announced the signing of Algeria striker Islam Slimani from Sporting Lisbon and allowed Gokhan Inler to leave the club.
Sunderland penned a deal for midfielder Didier Ndong from Lorient for a fee of £13.6million.
In the UAE, former Al Ain striker Asamoah Gyan completed a sensational move to former rival and current league holders Al Alhi.