The Netherlands will not feature at Euro 2016 after a 3-2 defeat to the Czech Republic ensured a miserable qualifying campaign ended in failure.
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The Dutch knew only victory would do in the Amsterdam Arena and even then they needed third-placed Turkey to lose to Iceland to clinch third place themselves and a play-off spot in Group A.
Goals from Pavel Kaderabek and Josef Sural, however, put the Czech Republic 2-0 up inside half an hour and while the visitors were reduced to 10 men when Marek Suchy was sent off before half-time, Holland failed to capitalise.
Instead, Robin van Persie, on as a substitute in the 38th minute, headed home an own goal to put the result beyond doubt, before Klaas-Jan Huntelaar and Van Persie scored late consolations.
It is the first time Holland have failed to qualify for a major tournament since they missed the 2002 World Cup and attention will swiftly turn to the future of head coach Danny Blind.
Wales finished their memorable Euro 2016 campaign on a winning note as Aaron Ramsey and Gareth Bale crowned a Cardiff party with second-half goals in a 2-0 victory over Andorra.
The win was not enough for Wales to win Group B as Belgium took top spot by beating Israel in Brussels.
But that will not matter to Welsh fans who had turned up in their thousands in a sell-out Cardiff City Stadium crowd to mark the nation reaching its first major tournament since the 1958 World Cup.
The party atmosphere was understandable as Wales have not qualified for anything since the days of John Charles and Ivor Allchurch.
Italy came from behind with two goals in the second half to beat Norway 2-1 in Rome on Tuesday and clinch first place in Group H of Euro 2016 qualifying.
Turkey ended Holland's hopes of qualifying for Euro 2016 and sealed progress themselves as Selcuk Inan's late winner snatched a 1-0 win against Iceland.
Croatia edged Malta 1-0 on Tuesday evening and moved into second place in Group H to qualify automatically for Euro 2016.
Bulgaria ended their unsuccessful Euro 2016 qualifying campaign with a 2-0 win at home to Azerbaijan.
Islambek Kuat's second-half strike earned Kazakhstan a 1-0 victory over Latvia in their final Euro 2016 Group A qualifier in Riga on Tuesday night.
Bosnia-Herzegovina fought back from a goal down to beat Cyprus 3-2 in their final Euro 2016 qualifier in Nicosia to finish third in Group B and clinch a play-off place.
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